Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Goat Acetylcholinesterase (also known as AChE) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Goat AChE antibody. AChE present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Goat AChE Antibody is added and binds to AChE in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated AChE antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Goat AChE. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm